Cannabis can alter male sperm DNA changes associated with autism

Parental cannabis use has been associated with adverse neurodevelopmental outcomes in offspring, but how such phenotypes are transmitted is largely unknown. Using reduced representation bisulphite sequencing (RRBS), we recently demonstrated that cannabis use is associated with widespread DNA methylation changes in human and rat sperm. Discs-Large Associated Protein 2 (DLGAP2), involved in synapse organization, neuronal signaling, and strongly implicated in autism, exhibited significant hypomethylation (p < 0.05) at 17 CpG sites in human sperm. We successfully validated the differential methylation present in DLGAP2 for nine CpG sites located in intron seven (p < 0.05) using quantitative bisulphite pyrosequencing. Intron 7 DNA methylation and DLGAP2 expression in human conceptal brain tissue were inversely correlated (p < 0.01). Adult male rats exposed to delta-9-tetrahydrocannabinol (THC) showed differential DNA methylation at Dlgap2 in sperm (p < 0.03), as did the nucleus accumbens of rats whose fathers were exposed to THC prior to conception (p < 0.05). Altogether, these results warrant further investigation into the effects of preconception cannabis use in males and the potential effects on subsequent generations.

https://pubmed.ncbi.nlm.nih.gov/31451081/

 

Like this article?

Share on Facebook
Share on Twitter
Share on Linkdin
Share on Pinterest

Leave a comment

Related Posts

Federal Regulations of Cannabis for Public Health in the United States

Millions of Americans use cannabis in moderation each month without any problems. However, evidence is mounting of harmful physical and mental health effects associated with heavy or long-term regular cannabis use. At the same time, existing legal markets in the United States are making high-potency cannabis products available to adult consumers. Recent international recommendations suggest

Vaping May Open the Door to Cannabis Use in Teens

Adolescents who used e-cigarettes were significantly more likely to become new smokers of cannabis shortly thereafter, a cohort study found. Cannabis-naive adolescents reporting ever vaping at baseline were more than twice as likely to report also using cannabis when surveyed a year later (adjusted RR 2.57 vs nonusers of e-cigarettes, 95% CI 2.04-3.09), reported Ruoyan

Clinical management of cannabis withdrawal

Cannabis withdrawal is a well-characterized phenomenon that occurs in approximately half of regular and dependent cannabis users after abrupt cessation or significant reductions in cannabis products that contain Δ9-tetrahydrocannabinol (THC). This review describes the diagnosis, prevalence, course and management of cannabis withdrawal and highlights opportunities for future clinical research. Read the full study published in

Association of cannabis potency with mental ill health and addiction: a systematic review

Cannabis potency, defined as the concentration of Δ9-tetrahydrocannabinol (THC), has increased internationally, which could increase the risk of adverse health outcomes for cannabis users. We present, to our knowledge, the first systematic review of the association of cannabis potency with mental health and addiction (PROSPERO, CRD42021226447). We searched Embase, PsycINFO, and MEDLINE (from database inception

Highly potent weed creating marijuana addicts worldwide, study says

Higher concentrations of tetrahydrocannabinol or THC — the part of the marijuana plant that makes you high — are causing more people to become addicted in many parts of the world, a new review of studies found. Read the full article from CNN here: https://amp.cnn.com/cnn/2022/07/25/health/marijuana-potency-addiction-study-wellness/index.html For more information on the International Academy on the Science

Scroll to Top